EPITH SODIUM CHAN GAMMA; RBX-50UG

Application

Western blot: 1-10 µg/mL using ECL. 83-90kDa bands; Masilamani et al 1999

ELISA: 1:10,000-1:100,000 using 1 mg/mL control peptide coated on well.

Optimal working dilutions must be determined by the end user.

Detect Epithelial Sodium Channel-γ using this Anti-Epithelial Sodium Channel-γ Antibody validated for use in ELISA & WB.

Immunogen

A 14 amino acid peptide sequence near the N-terminus of rat ENaC gamma (Linguela et al. 1993; Canessa et al.

1993; Snyder et al. 1994; Waldmann et al. 1995; Kreutz et al. 1997; Voilley et al.

1994; McDonald et al. 1994; Ludwig et al. 1998; Garty & Palmer 1997).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA.

Specificity

Recognizes rat Epithelial Sodium Channel gamma (ENaC gamma). The immunogen shows no significant sequence homology with other ENaC subunits.

SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 92% in human, 80% in rabbit. Reactivity with other species has not been confirmed.